The PCR technique is utilized to replicate DNA in the lab and requires repeated heating of the sample to near the boiling point of water. What is the name of the enzymes required for this technique (it adds the right nucleotides to form the new DNA polymer during DNA replication) and what type of organism does the specific enzyme used in PCR come from? DNA polymerase from a hyperthermophile DNA gyrase from a thermophile RNA polymerase from a mesophile Helicase from a psychrophile None of these are correct The PCR technique is utilized to replicate DNA in the lab and requires repeated heating of the sample to near the boiling point of water. What is the name of the enzymes required for this technigue (it adds the right nucleotides to form the new DNA polymer during DNA replication) and what type of organism does the specific enzyme used in PCR come from? DNA polymerase from a hyperthermophile DNA gyrase from a thermophile RNA polymerase from a mesophile Helicase from a psychrophile None of these are correct
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DNA polymerase is responsible for adding the correct nucleotides to form the new DNA polymer during DNA replication. Show more…
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As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR, template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No ligase is required in PCR, but it is necessary for DNA replication within the cell. If DNA ligase is essential for DNA replication in a cell, why isn't it necessary in PCR? (3 marks) Hint: Think about what the role of ligase is normally in replication.
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