Question

Fluorescence Cycles II.5 II.9 PGD 4 II.8 Threshold PGD 3 II.6

          Fluorescence
Cycles
II.5
II.9
PGD 4
II.8
Threshold
PGD 3
II.6
        
Fluorescence
Cycles
II.5
II.9
PGD 4
II.8
Threshold
PGD 3
II.6

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Biology for AP Courses
Biology for AP Courses
Julianne Zedalis, John Eggebrecht
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The results obtained with the Real-Time PCR procedure conducted on parents and PGDs of family 2 give us several important pieces of information. The results obtained here represent a quantification of Dystrophin DNA molecules initially present in the biopsies. The PCR amplification leads to an exponential accumulation of fluorescent nucleotides measured after each cycle. From these results, we can conclude that PGD3 is non-affected and that PGD4 is affected. In family 2, the individual II.8 often complains of some muscle weakness. Looking at the result obtained for this family, you can say that it makes sense since this individual has lower levels of dystrophin than others. We cannot comment on this observation based on the results we have. In addition to all other information deduced before, we can also conclude from this result that PGD4 is a male carrier with two X chromosomes. PGD4 and II.8 are female carriers with substantial inactivation of the non-mutated X chromosome. PGD3 is most likely a male fetus. Individuals II.5 and II.9 show a substantial inactivation of the non-mutated X chromosome. 11.5 PGD4 11.8 11.9 Fluorescence Threshold PGD3 11.6 Cycles
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Transcript

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00:01 Hello students in this video i will be answering multiple questions regarding genetics and molecular biology.
00:09 To begin with the first question, mendel's experiment with emasculation inward, removing the anthers of 1 p plant and manually transferring pollen from another plant to the emasculated plant to create a cross.
00:25 To demonstrate that emasculation was not the reason why the wrinkle seed shape, disappeared in f1 generation, mendel performed a control experiment where he did not emasculate the flower but he covered the anthers so that there will be no self fertilization in that particular flower.
00:45 And he crossed that flower with pollen from another plant and the results showed that f1 generation still had only round seeds indicating that emasculation was not the reason for for the disappearance of wrinkled seed shape.
01:05 The second question, testcross used plants that were homozygous recessive because they allowed mendel to determine the genotype of the f2 plant.
01:18 If the f2 plant was heterozygous, then crossing it with homozygous resettive plant would yield a result of 1 -1 ratio of dominant to racive alleles.
01:31 In the offspring.
01:32 On the other hand, if mendel had used a homozygous dominant plant for test cross, all the offspring would have a dominant phenotype, making it impossible to determine the genotype of the f2 plant.
01:49 Moving on to the third question, variable expressivity is a term used to describe the phenomena where a genetic trite is present in different individuals to varying degrees...
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