The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. | Enzyme | Target sequence | Cleavage | |---|---|---| | EcoRI | 5' GAATTC 3' 3' CTTAAG 5' | 5' G AATTC 3' 3' CTTAA G 5' | | EcoRV | 5' GATATC 3' 3' CTATAG 5' | 5' GAT ATC 3' 3' CTA TAG 5' | | HaeIII | 5' GGCC 3' 3' CCGG 5' | 5' GG CC 3' 3' CC GG 5' | | HindIII | 5' AAGCTT 3' 3' TTCGAA 5' | 5' A AGCTT 3' 3' TTCGA A 5' | | PpuMI | 5' RGGWCCY 3' 3' YCCWGGR 5' | 5' RG GWCCY 3' 3' YCCWG GR 5' | Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' GAAGATATCCTCGC 3' HindIII EcoRI HaeIII EcoRV 5' TCCAAGCTTGAATTC 3' EcoRV EcoRI HindIII HaellI
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- EcoRI: 5' GAATTC 3' - Not present - EcoRV: 5' GATATC 3' - Present - HaeIII: 5' GGCC 3' - Not present - HindIII: 5' AAGCTT 3' - Not present Therefore, EcoRV would cleave the first DNA sequence. Show more…
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The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme Target sequence Cleavage EcoRI 5' GAATTC 3' 5' G AATTC 3' 3' CTTAAG 5' 3' CTTAA G 5' EcoRV 5' GATATC 3' 5' GAT ATC 3' 3' CTATAG 5' 3' CTA TAG 5' HaeIII 5' GGCC 3' 5' GG CC 3' 3' CCGG 5' 3' CC GG 5' HindIII 5' AAGCTT 3' 5' A AGCTT 3' 3' TTCGAA 5' 3' TTCGA A 5' PpuMI 5' RGGWCCY 3' 5' RG GWCCY 3' 3' YCCWGGR 5' 3' YCCWG GR 5' Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' GCCAATTGAAGCTT 3' 5' AGGCCGAATTCGC 3' HaeIII HindIII HindIII EcoRI EcoRI HaeIII EcoRV EcoRV
Sri K.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme | Target sequence | Cleavage EcoRI | 5' GAATTC 3' / 3' CTTAAG 5' | 5' G AATTC 3' / 3' CTTAA G 5' EcoRV | 5' GATATC 3' / 3' CTATAG 5' | 5' GAT ATC 3' / 3' CTA TAG 5' HaeIII | 5' GGCC 3' / 3' CCGG 5' | 5' GG CC 3' / 3' CC GG 5' HindIII | 5' AAGCTT 3' / 3' TTCGAA 5' | 5' A AGCTT 3' / 3' TTCGA A 5' PpuMI | 5' RGGWCCY 3' / 3' YCCWGGR 5' | 5' RG GWCCY 3' / 3' YCCWG GR 5' Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' GCCAATTGAAGCTT 3' HindIII HaeIII EcoRI EcoRV 5' AAAGCTTGTGATATC 3' EcoRV EcoRI HindIII HaeIII
Adi S.
The following table shows where different restriction enzymes cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme: EcoRI Target sequence: 5' GAATTC 3', 3' CTTAAG 5' Cleavage: 5' G AATTC 3', 3' CTTAA G 5' Enzyme: EcoRV Target sequence: 5' GATATC 3', 3' CTATAG 5' Cleavage: 5' GAT ATC 3', 3' CTA TAG 5' Enzyme: HaeIII Target sequence: 5' GGCC 3', 3' CCGG 5' Cleavage: 5' GG CC 3', 3' CC GG 5' Enzyme: HindIII Target sequence: 5' AAGCTT 3', 3' TTCGAA 5' Cleavage: 5' A AGCTT 3', 3' TTCGA A 5' Enzyme: PpuMI Target sequence: 5' RGGWCCY 3', 3' YCCWGGR 5' Cleavage: 5' RG GWCCY 3', 3' YCCWG GR 5' Which restriction enzyme(s) would cleave the following sequence? Choose all correct options. 5'-TCCAAGCTTGAATTC-3' 3'-AGGTTCGAACTTAAG-5'
Madhur L.
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