Question

Wash your mouth well. With the toothpick given gently remove a few cheek Cells and mount them on a slide using the procedure: 1) fix in absolute alcohol. 2) Transfer sections to 90%, 70%, 50%, 25% alcohol and finally water. 3) Place in a drop of hematoxylin 4) Transfer to acid alcohol to destain 5) Blue in ammonia 6) Place in 70% alcohol 7) Counterstain with eosin (1-2 minutes) 8) Dehydrate 70%, 90%, & 100% alcohol. 9) Clear in xylol 10) Mount in glycerol 11) Observe specimen under the light microscope. 1) What is the function of xylol? 2) What is the meaning of destaining? Note : When you stain the specimen should be in the medium the stain is in ( if the stain is in water the specimen also should be in the water)

          Wash your mouth well. With the toothpick given gently remove a few cheek Cells and mount
them on a slide using the procedure:
1) fix in absolute alcohol.
2) Transfer sections to 90%, 70%, 50%, 25% alcohol and finally water.
3) Place in a drop of hematoxylin
4) Transfer to acid alcohol to destain
5) Blue in ammonia
6) Place in 70% alcohol
7) Counterstain with eosin (1-2 minutes)
8) Dehydrate 70%, 90%, & 100% alcohol.
9) Clear in xylol
10) Mount in glycerol
11) Observe specimen under the light microscope.

1) What is the function of xylol?
2) What is the meaning of destaining?

Note :
When you stain the specimen should be in the medium the stain is in ( if the stain is in water
the specimen also should be in the water)

Wash your mouth well. With the toothpick given gently remove a few cheek Cells and mount
them on a slide using the procedure:
1) fix in absolute alcohol.
2) Transfer sections to 90%, 70%, 50%, 25% alcohol and finally water.
3) Place in a drop of hematoxylin
4) Transfer to acid alcohol to destain
5) Blue in ammonia
6) Place in 70% alcohol
7) Counterstain with eosin (1-2 minutes)
8) Dehydrate 70%, 90%, & 100% alcohol.
9) Clear in xylol
10) Mount in glycerol
11) Observe specimen under the light microscope.

1) What is the function of xylol?
2) What is the meaning of destaining?

Note :
When you stain the specimen should be in the medium the stain is in ( if the stain is in water
the specimen also should be in the water)

Added by Xavier M.

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