There are two questions in this quiz. Make sure you complete...

There are two questions in this quiz. Make sure you complete both (#2 is on the second page). You can print this out and complete the questions directly on here, scan and submit to the drop box or do the questions on a separate sheet of paper, scan and submit. Please provide all your work for your solutions in order to receive full marks. 1. The following is a strand of template DNA of a gene; 3' TACCCATAGCCAACT 5' Draw a labelled diagram with the first two codons of the mature mRNA loaded into the P and A windows of a ribosome. Indicate with an arrow, the direction of amino acid transfer that would occur, including labelled 5' and 3' ends. The windows should also be loaded with the appropriate tRNA molecules and their amino acids. mRNA: Diagram:

Transcript

00:01 So you're given a strand of template dna of a gene.

00:06 So the question asks you to write down mrna sequence first, and then draw a label diagram with the first two codon of the material and a window, and also indicate the direction of the mrna and also use an arrow to indicate the direction of amino acid transfer that would occur.

00:24 So i use black color to represent the template dna strand.

00:29 So we know that during transcription, the template strand of dna is used as a template to produce new mrna.

00:36 The new mrna is complementary to the template string.

00:40 So this gives us two information.

00:42 First of all, the direction or orientation of the mra is the opposite to the dna.

00:48 So as you can see, i use red color to write down the mrn.

00:51 So we knew that it's complementary sequence.

00:54 So the five prime end of the mrn is the opposite to the dna, which is on the left side.

01:02 And the 3 prime end of mrna is on the right side.

01:05 So you can see the two strands are opposite orientation.

01:08 Now, it also followed the base pair rule when it comes to complementary strength.

01:14 Whenever there is a t in dna, and you're gonna pair up with an a in mra.

01:20 And whenever there's a a, now, since there's no t in mra, you have to replace that with a ura cell.

01:27 Now, c also pair with g.

01:29 So according to the base pair rule, i have written down the mrna c for you in red color already.

01:34 All right, so now the second part, you want to draw a diagram of translation where the m rna is being translated into polypeptide by ribosone.

01:43 So the line on the left side of the first code on the line on the right after the last code is called the utr region, untranslated region.

01:56 So 5 prime uti is where the ribosome binds, and it actually scans through the mrna to translate.

02:02 So the ribosome translates mrna in the format of codon.

02:07 A codon is every three nucleotide.

02:10 So aug is the first codon.

02:13 So a codon will give you a specific amino acid.

02:17 So this information can be found by the codon usage table that's given by the question.

02:22 Now the very first codon of the peptide is called the stark codon.

02:28 It is also a methionine.

02:29 So aug is the very first codon of the peptide.

02:32 Of this polypeptide.

02:34 So it gives you amino acid methionine.

02:37 So i'm using green color to write down the antichotone on t -r -n -a.

02:43 The anti -codone is, again, complementary to the codon on an m -r -n -a.

02:47 So as you can see, the first anticoad on t -r -n -a is from 5 to 3, c -a -u...

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