Thin layer Chromatography Biological molecules can be...

Thin layer Chromatography Biological molecules can be separated by using chromatographic techniques. The diagram below shows the separation of several spinach leaf pigments by paper chromatography. Using the diagram below, answer the following questions: Solvent Line Carotene (yellow orange) Xanthophyll (yellow) Chlorophyll a (blue green) Chlorophyll b (greenish yellow) Pigment Extract (origin)

Transcript

00:01 Hi, so the answer is chromatography, chromatography, chromatography.

00:08 Basically this chromatography, it is a technique of separation, it is a technique of separation of biomolecules, separation of bio molecules, involves in sampling mix, involves in the sampling a mixture containing the biomolecules which being dissolved which is being dissolved in a mobile phase dissolved in a mobile phase due to their differential absorption over an absorbent medium over an absorbent adsorbent medium so coming up to the answer for the first part of the question is that the v -shaped here the v -shaped tip of the paper v -shaped tip of the paper is placed in the chromatography.

01:13 It is placed in the chromatography solvent and act as a wick to draw.

01:23 Act as a wick to draw to their relative solubility to their relative solubility and molecular weight and the molecular molecular weight so here the paper is allowed to remain here the paper which is allowed to remain in the solvent it will remain in the solvent until the uppermost, until the upper most pigment band, upper most, pigment band near to the top of the paper.

02:08 Near to the top of the paper.

02:11 So here the factors, basically here the factors that are involved in the separation that are involved in the separation.

02:23 Of the pigment in the separation of the pigment in the lab, pigment in the lab are solubility are solubility size, size of the particles, size of the particles and their attractiveness to the paper and their attractiveness to the paper which is being involved in the separation of the pigment in the separation of the pigment.

03:03 Now coming up to the second part of the question, so the answer for the second part is that photosynthesis, photosynthesis is the process of capturing capturing light energy through light energy through pigments light energy through the pigment now this pigment absorb this pigment basically it absorb at it absorbed at different wavelength using an example of photosystem 2 using an example of photosystem 2 which is absorbing at 680 nm...

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