Hydroxylamine is a mutagenic chemical. Use the codon table to help determine the effect of hydroxylamine. Step 5: Determine how treatment with hydroxylamine will alter each DNA stop codon and the resulting mRNA stop codon. In the last step, the DNA equivalent of each mRNA stop codon was determined. The mRNA and matching DNA stop codons are listed in the table. Also, recall from a previous step that hydroxylamine exposure results in the replacement of a C with a T and a G with an A. Determine how the reaction of DNA with hydroxylamine will alter each stop codon in the DNA and the mRNA. Place the DNA and mRNA stop codons after treatment with hydroxylamine under each of the original mRNA stop codons. The DNA stop codons also include the complementary strand of DNA. mRNA stop codon UAA UAG UGA DNA stop codon TAA ATT TAG ATC TGA ACT Hydroxylamine treatment -------- -------- -------- DNA after hydroxylamine TAA ATT TAG ATC TGA ACT mRNA after hydroxylamine UAA UAG UGA TAA ATT UGA
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Use the DNA sequence below to answer the following questions: 3' TACTTACTCGTCAACCTT 5' a) Write the corresponding mRNA coding sequence. /1 b) Use the table below to determine the amino acid sequence; /1 c) A nucleotide substitution occurs in the DNA template at position 14, where adenine becomes thymine. Write the new mRNA coding sequence and amino acid sequence that would result from this substitution. /2 d) What type of mutation would this yield? /1
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The following mRNA sequence includes the start codon of an mRNA transcript: 5'-AUGCGUGAC-3' The underline U is mutated to an A. Use the genetic code below to answer the following questions. Please include the letter for each part in your answer. a. What term describes the replacement of one nucleotide with another? (2 pts) b. What term describes the molecular effect of the mutation on the nucleotide sequence? (2 pts) c. What term describes the molecular effect of the mutation on the amino acid sequence? (2 pts) d. What term describes the most likely phenotypic effect of the mutation on the activity of the associated polypeptide? e. What is one general class of mutagen most likely to cause the mutation? (2 pts)
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