00:01
Hello students, we have to describe the three main steps to clone a gene into an organism.
00:20
First step if we see it will be isolation of the gene of interest.
00:37
In this the cloning of gene is to for cloning of the gene into an organism we have to isolate the gene of interest from the source organism.
00:50
This could be done using various techniques such as pcr, restriction enzyme, digestion or hybridization.
01:14
Once the gene is isolated it needs to be purified and amplified.
01:19
It needs to be purified and amplified to obtain the enough dna for further manipulation.
01:43
Step two will be insertion of the gene into a vector which is the dna molecule that carry the gene into the host organism.
02:18
Here the dna molecule it carries the gene into the host organism.
02:40
The vector must have certain features such as promoter, it must have promoter to drive the gene expression, selectable marker to identify the cells that have to be take up by the vector and replication origin to allow the vector to replicate within the host cell...