Transcription and translation worksheet Using the provided...

Transcription and translation worksheet Using the provided code, fill in the following DNA and protein code. Use the single letter abbreviation to translate the RNA code into the protein code. Please recall which codon translation actually begins translation. Make sure you properly label the 5’ and 3’ ends of the DNA sequences of the template and non-template strands and N-terminus and C-terminus of the polypeptide/amino acid translating. DNA Template Non-template RNA mRNA: 5’ GAU GGG CCC AUG GAA CUC AUA AAG GAA UGC CAA CAA AAG AUC GAA AGC UAG CGG AAA Protein Amino acid

Transcript

00:01 Okay, so this question is basically two parts.

00:04 Essentially, we are given an mrna sequence here, and we can come up with the dna template and non -template, as well as the amino acid sequence.

00:14 So let's go ahead and do the template dna and then non -template dna first.

00:20 So remember that dna has two strands, and then the mrna codes off of one, is complementary to one strand.

00:31 The thing is, if the gene of interest is on this strand, this is what's known as the non -template strand, otherwise known as the coding strand, because this has the actual gene.

00:52 The complementary to the coding strand or the non -teplit strand is the template strand, because that's what the mrnas is basically getting created too.

01:02 That's the template.

01:03 So the sequence of the mrna here and the coding sequence is actually going to be the same, with the exception, of course, that dna doesn't have urocells, it has thymines.

01:19 So if the 5 prime is gau for the mrna, then 5 prime for the template strat should be gat.

01:28 All right.

01:31 So we'll go ahead and fill that out.

01:35 And all right, so i've gone ahead and filled out the complementary text.

01:40 Template strand based off the mrna here.

01:44 And i've moved the mrna sequence up, so it's in the same line.

01:48 So in the black we have the template strand, and in blue we have the non -templit strand.

01:56 And remember, the template strand is the same sequence as the mra, with the exception of the urusole being replaced by thymies, and then the non -teplice strand is complementary to the template strand.

02:08 So let's go ahead and go to the protein.

02:10 Not to determine what codon translates to, which i mean, i ask, excuse, we can use this, code on chart.

02:25 Let's see this.

02:27 So our first codon is g -a -u, right? so to use this, we first look at the first letter of the codine, g, then a, up here, and then you...

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