00:01
All right, so in this assignment, it's basically transcribing and translating two different sequences.
00:07
So let's go ahead and get started with the first sequence.
00:10
So we have the sequence in black.
00:12
That is the sequence given to us.
00:14
And the question is asking, what is the sequence of the complementary sense strand of the dna? so basically, if this were to be double -stranded dna, what would the complement be to that? so, again, it would be t -t -a, t -g -g -g -a, c, g -g -t -t, t.
00:37
Okay, and the 3 -prime would be on this end.
00:40
So that would be the complementary dna strand.
00:45
So that's dna.
00:46
Let's clarify that.
00:50
Now, the next question is, what would the sequence be of the mrna? so the mrna is going to be complementing this.
01:03
Now remember, rna does not have thymines, they have uracill, so not t's, but u.
01:09
U.
01:10
So complement to that, it'll be u, u, u, a, u, g, g, u, c, i could be written better, c, g, u, u.
01:33
3 prime end here, 5 prime end here, right? this is going to be complementary to that.
01:40
And this makes sense because rna polymerase goes from on the template strand, 3 prime to 5 prime.
01:46
So it spits out the mrna in a 5 prime to 3 prime fashion.
01:50
Okay.
01:50
So this is the rmrna.
01:54
And then what are the first three amino acids that result from this? so let's go with blue.
02:01
Okay.
02:02
So then what is the amino acid sequence? so to divide up the codons, we have this.
02:14
Codon, codon, codon, codon.
02:16
So they're asking for the first three amino acids.
02:19
And so we can look up the amino acid chart, which you should be able to search for that very easily.
02:26
And i'm so sorry.
02:27
I just realized the question is a little more nuanced than just a simple thing.
02:36
So actually, so we have this mrna sequence, right? however, however, there's always a sequence, a codon, known as the start codons.
02:49
So all proteins that are being translated from mrna have begin with the start codon, which is aug, start codon, right? so that is going to be, this is going to be our start codon.
03:06
These two are not translated.
03:09
So this is the beginning of our frame, this is the next frame, this is the next frame.
03:13
So really, this sequence, and obviously u is not translated, so this sequence really has only three amino acids.
03:20
So the first amino acid, aug, is going to always be thionine, met, followed by g -u, which is going to be valine, followed by c -gu, which is going to be argonine.
03:42
Okay, so this is the three amino acid sequence out is derived from this mrna sequence.
03:50
Okay, so the next part of the question.
03:52
So the same first part.
03:54
So the next part is what if the first amino acid, i'm sorry, the first cytosine in the template dna strand is deleted...