In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (amp?). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized below. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria. NUTRIENT AGAR PLATES No Ampicillin Ampicillin Wild-type E. coli I II E. coli and amp? plasmid III IV
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Step 1: Plate I (wild-type, no plasmid, no ampicillin) shows confluent growth — outcome: bacteria grow extensively. Show more…
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In a transformation experiment, a sample of competent bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic, ampicillin (ampr). Plasmid was not added to a second sample of bacteria. The samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of bacterial growth are summarised below. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria. No Ampicillin Bacteria Ampicillin Bacteria No Ampicillin Ampicillin Bacteria + amp' plasmid Bacteria + amp' plasmid Which are the plate(s) that have ONLY ampicillin-resistant bacteria growing? (6 marks) (a) Plate I only (b) Plate III only (c) Plate IV only (d) Plates I and II (e) Plates I and III
Farhan A.
5. In a transformation experiment containing two samples, the first sample of E. coli was mixed with a plasmid containing the ampR gene. The plasmid was not added to the second sample of E. coli (i.e. the wild type E. coli bacteria figure at right). Both samples were plated on nutrient agar, half of which were supplemented with the antibiotic, ampicillin, and the other half were not. The results of E. coli growth are shown. Dots indicate growth of bacteria. Answer these questions based on this experiment: a. Plates that have ampicillin-resistant bacteria growing would be which plate(s)? I, II, III, IV - list your answer(s) b. Why is there no growth on plate II? c. (Circle the correct answer) Plates I and III were included in the experimental design in order to - demonstrate that the plasmid can lose its ampR gene. - demonstrate that the plasmid is needed for E. coli growth. - demonstrate that E. coli cultures were viable. - prepare the E. coli for transformation.
Qbs E.
A geneticist is working with a new bacteriophage called phage Y3 that infects $E$ coli. He has isolated eight mutant phages that fail to produce plaques when grown on $E$. coli strain K. To determine whether these mutations occur at the same functional gene, he simultaneously infects $E .$ coli $\mathrm{K}$ cells with paired combinations of the mutants and looks to see whether plaques are formed. He obtains the following results. (A plus sign means that plaques were formed on $E$ coli $\mathrm{K}$; a minus sign means that no plaques were formed on $E$ coli K.) (TABLE CAN'T COPY) a. To how many functional genes (cistrons) do these mutations belong? b. Which mutations belong to the same functional gene?
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