Transgenic fusions between a gene and a reporter are used to determine whether a gene is expressed. Which of these components allows the researcher to work with living specimens, and why? The lacZ protein is often used to screen cells in culture through green screening. It requires the substrate X-gal to produce a cyan product that may have not toxic effects for the living organisms. The green fluorescent protein (GFP) is toxic and requires a substrate. The lacZ protein is often used to screen cells in culture through green screening. It requires the UV light to produce a blue product that may have toxic effects for the living organisms. The green fluorescent protein (GFP) is toxic and requires no substrate. The lacZ protein is often used to screen cells in culture through blue-white screening. It requires the substrate X-gal to produce a blue product that may have toxic effects for the living organisms. The green fluorescent protein (GFP) is not toxic and requires no substrate. The lacZ protein is often used to screen cells in culture through blue-white screening. It requires the UV light to produce a green product that may have no toxic effects for the living organisms. The green fluorescent protein (GFP) is toxic and requires no substrate.
Added by Deborah M.
Close
Step 1
This component allows the researcher to work with living specimens because it can be easily visualized under a fluorescence microscope without causing harm to the organism. GFP can be fused to the gene of interest, and Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 77 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Reporter genes such as GFP, which produces green fluorescent protein in flashes when activated, allow for single-cell examination of gene expression, as opposed to the reporter gene lacZ, which shows the population's average response. What kinds of questions can be answered better using a population-level approach? a. Timing and coordination of gene activation under changing environmental conditions b. Movement of pathogens in host animals c. Activation of certain types of genes under changing environmental conditions d. Where specific proteins are located within cells
Dave K.
Which of the following is an example of a transgenic organism that is used for bioremediation? A fish infused with a gene from another fish species that accelerates growth rate A plant infused with a bacterial gene for a protein to break down the environmental toxin mercury A bacteria infused with a human insulin gene A cow infused with a human milk protein gene
Maitreya E.
Which of the following is used as a reporter gene in recombinant DNA work with bacteria as host cells? a. rRNA b. Green fluorescent protein c. Antibiotic sensitivity d. Ability to make ornithine e. Vitamin synthesis
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD