tRNAs for all 20 amino acids have been cloned. Mutagenesis and/or engineering of each plasmid- borne tRNA so that their anticodon should recognize the amber codon resulted in only 13 (of the possible 20) amber suppressor tRNAs. Provide plausible explanations why 7 suppressor tRNAs failed to be isolated.
Added by Jenny M.
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This could be due to the specific structure and properties of the amino acid, making it difficult to find a compatible anticodon sequence. Show more…
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