00:01
All right.
00:02
So today we're going to be going over whether a, when you're sterilizing media in an autoclave, if the microbes are killed by the high pressure within the autoclave.
00:12
So to answer this true or false, i would say that this is false, but let's go into that a little bit more.
00:24
So it's not exactly the pressure in the autoclave that's doing the killing.
00:30
It's actually going to be temperature.
00:32
And the reason for this is that the high pressure in the autoclave is going to allow for the boiling point of water to be increased, honestly, by quite a bit.
00:47
Basically, a good way to think about this is in the inverse.
00:53
Hopefully, you've traveled a little bit, and you know that whenever you are doing any type of cooking up on the mountains, or at any place of high elevation, water is going to boil a lot quicker.
01:09
It's going to boil at a lower temperature.
01:12
And the reason for this is that boiling is actually directly proportionate to the surrounding pressure.
01:21
Because what it is is an equalization of the vapor pressure within the water, equaling out to the surrounding pressure, and then the water vapor is able to escape, and that's what boiling is.
01:36
So in the autoclave, by going ahead and, let's go and just draw an autoclave here, by the pressure going up, so will the boiling point.
01:54
And that's basically going to look like this.
01:58
Let me try that again.
02:00
So where we have pressure, and boiling point.
02:06
It's going to go like that.
02:09
And the key one that we're going to be looking at is going to be at 15 psi...