Question

2. (4 marks) As an analytical chemist performing the FRAP assay, you need to prepare a buffer of pH 3.67. You find the following acid/base pairs to prepare your buffer: • hydrofluoric acid/sodium fluoride • acetic acid/sodium acetate • 4-Morpholineethanesulfonic acid (MES)/4-morpholineethanesulfonate Calculate the ratio of conjugate base to conjugate acid ([A?]/[HA]) required to make the buffer solution for each pair. Select the best acid/base combination to prepare the buffer in real life and justify your choice. For questions 3 and 4, you will be asked to design procedures using the following materials. You may or may not need all of these chemicals or glassware. • Standard equipment basket from your locker • FeCl?·6H?O • FeSO?·7H?O • 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) • Buffer (pH 3.67) – The buffer solution at this pH will be prepared for you. • 0.040 M HCl solution – The acid solution at this pH will be prepared for you • One 10 mL volumetric flask • 50 mL volumetric flasks • 5 mL volumetric pipette • 100-1000 µL micropipette • 25 mL grad cylinder 3. (3 marks) For this experiment, you will need to prepare 60 mL of the FRAP solution. To this end, make 10 mL of a 10 mM TPTZ solution and at least 5 mL of a 20 mM Fe³? solution. Mix your solutions to form a volumetric ratio of 1:1:10 of the TPTZ: Fe³?:Buffer solutions. Fill the table below while designing your procedure, and remember to consider the need for quantitative transfers. Staple this prelab with the completed table to your lab notebook. Reagent | Mass (g) | Solvent | Volume (mL) | Glassware used N/A | N/A 4. (3 marks) You will need to create a calibration curve with six absorbance data points ranging from 0 to 0.6. Using 50 mL volumetric flasks and 5 mL of the FRAP reagent in each flask, design a procedure that allows you to create this curve. Your procedure must describe whether a stock solution is needed or not, and if needed, describe how to prepare the stock solution. Please Note: For information regarding calibration curves, see the lab manual: Introduction to Statistics and/or UV-Nitrite.

2. (4 marks) As an analytical chemist performing the FRAP assay, you need to prepare a buffer of pH 3.67. You find the following acid/base pairs to prepare your buffer:
• hydrofluoric acid/sodium fluoride
• acetic acid/sodium acetate
• 4-Morpholineethanesulfonic acid (MES)/4-morpholineethanesulfonate
Calculate the ratio of conjugate base to conjugate acid ([A?]/[HA]) required to make the buffer solution for each pair. Select the best acid/base combination to prepare the buffer in real life and justify your choice.

For questions 3 and 4, you will be asked to design procedures using the following materials. You may or may not need all of these chemicals or glassware.
• Standard equipment basket from your locker
• FeCl?·6H?O
• FeSO?·7H?O
• 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ)
• Buffer (pH 3.67) – The buffer solution at this pH will be prepared for you.
• 0.040 M HCl solution – The acid solution at this pH will be prepared for you
• One 10 mL volumetric flask
• 50 mL volumetric flasks
• 5 mL volumetric pipette
• 100-1000 µL micropipette
• 25 mL grad cylinder

3. (3 marks) For this experiment, you will need to prepare 60 mL of the FRAP solution. To this end, make 10 mL of a 10 mM TPTZ solution and at least 5 mL of a 20 mM Fe³? solution. Mix your solutions to form a volumetric ratio of 1:1:10 of the TPTZ: Fe³?:Buffer solutions. Fill the table below while designing your procedure, and remember to consider the need for quantitative transfers. Staple this prelab with the completed table to your lab notebook.

4. (3 marks) You will need to create a calibration curve with six absorbance data points ranging from 0 to 0.6. Using 50 mL volumetric flasks and 5 mL of the FRAP reagent in each flask, design a procedure that allows you to create this curve. Your procedure must describe whether a stock solution is needed or not, and if needed, describe how to prepare the stock solution.
Please Note: For information regarding calibration curves, see the lab manual: Introduction to Statistics and/or UV-Nitrite.

2. (4 marks) As an analytical chemist performing the FRAP assay, you need to prepare a buffer of pH 3.67. You find the following acid/base pairs to prepare your buffer:
• hydrofluoric acid/sodium fluoride
• acetic acid/sodium acetate
• 4-Morpholineethanesulfonic acid (MES)/4-morpholineethanesulfonate
Calculate the ratio of conjugate base to conjugate acid ([A?]/[HA]) required to make the buffer solution for each pair. Select the best acid/base combination to prepare the buffer in real life and justify your choice.

For questions 3 and 4, you will be asked to design procedures using the following materials. You may or may not need all of these chemicals or glassware.
• Standard equipment basket from your locker
• FeCl?·6H?O
• FeSO?·7H?O
• 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ)
• Buffer (pH 3.67) – The buffer solution at this pH will be prepared for you.
• 0.040 M HCl solution – The acid solution at this pH will be prepared for you
• One 10 mL volumetric flask
• 50 mL volumetric flasks
• 5 mL volumetric pipette
• 100-1000 µL micropipette
• 25 mL grad cylinder

3. (3 marks) For this experiment, you will need to prepare 60 mL of the FRAP solution. To this end, make 10 mL of a 10 mM TPTZ solution and at least 5 mL of a 20 mM Fe³? solution. Mix your solutions to form a volumetric ratio of 1:1:10 of the TPTZ: Fe³?:Buffer solutions. Fill the table below while designing your procedure, and remember to consider the need for quantitative transfers. Staple this prelab with the completed table to your lab notebook.

Reagent | Mass (g) | Solvent | Volume (mL) | Glassware used
N/A | N/A

4. (3 marks) You will need to create a calibration curve with six absorbance data points ranging from 0 to 0.6. Using 50 mL volumetric flasks and 5 mL of the FRAP reagent in each flask, design a procedure that allows you to create this curve. Your procedure must describe whether a stock solution is needed or not, and if needed, describe how to prepare the stock solution.
Please Note: For information regarding calibration curves, see the lab manual: Introduction to Statistics and/or UV-Nitrite.

Added by Christie L.

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