Under the Microscope A surgical technologist working in the...

Under the Microscope A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. 1. Which type of bacteria would be used to test the autoclave? 2. Which characteristic of the species used determines whether sterility was achieved when autoclaved? 3. What must be done with the bacterial sample after it is processed in the sterilizer? 4. Do the bacteria used for biological testing of sterilizers pose any threat to humans? 5. If the biological indicator showed positive growth following the process in question 3, what would have to be done?

Transcript

00:01 Hello everyone so here in this question we are going to discuss about few parts that is given okay so the first part we need to discuss that which type of bacteria would be used to treat the test autoclave okay in autoclave process which bacteria should be used that we need to answer okay so first of all in the process of autoclave they basically it contains spores of spores of bacillus okay and mostly which type of bacteria we can use we can use the thermophilic bacterium okay thermophilic bacterium we can use thermophilic bacterium so this is the answer for the first part now we are going to discuss about the second part of the question that is which of the characters should you know present so that we can perform the autoclid so that must be the bacteria that is we are using in the process that should have the ability to ability to withstand to withstand heat and temperature these two should present okay heat and temperature that bacteria should withstand so this is the character now we will be discussing about the third part so what must be done must be done when bacteria are detected in the autoclive so in that process we should raise the temperature okay we should raise the temperature and uh in all by raising the temperature what will happen the bacteria that if will increase the temperature from the level they can withstand it will basically kill the bacteria right kill the bacterium so this is the answer for the third part and now we will be discussing about the poor part that do the bacteria that we are using in the sterilized process are they are harmful for humans or not they are threats for human or not so no they are not harmful for the humans they won't be causing any harmfulness to the humans so they are not harmful for humans right now we will be discussing over the last part that is if the biological indicator for positive growth the following process that what is the basically positive growth how how can we see? so and if there is positive growth then what we should do.

02:28 Okay, so that is the thing we are going to discuss.

02:31 So if there is any cloudiness, it means there is positive growth...

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