Use the growth curve below to answer the following questions. 1.2 Determine the generation time for bacterium A and B (0.5 point) b. Which bacterium has the longest lag? (0.5 pt) Which bacteria have induced the stringent response? (0.5 point) d. Which bacterium has the lowest yield? (0.5 point) e: Anna decides to grow bacterium A using continuous culture. Provide a diagram illustrating the growth of bacterium A as a function of the dilution rate on the plot below: Label the washout point on your diagram and indicate where the cells are in steady state? (1 point)
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Without the actual growth curve, it's impossible to determine the generation time for bacterium A and B. However, the generation time can be calculated by dividing the total time of growth by the number of generations. b. The bacterium with the longest lag would Show more…
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Question 3: From the data given below, calculate; (a) Draw the bacterial growth graph and show growth phases [15 point]. (b) The number of doublings over the total growth period [15 point]. (c) The generation time during the exponential growth phase [15 point]. Time (min) Cell number 0 3.85 x10^10 45 3.93 x10^10 90 4.47 x10^10 135 4.44 x10^10 180 7.60 x10^10 270 1.36 x10^11 360 2.98 x10^11 450 5.44 x10^11 540 7.50 x10^11 630 8.26 x10^11 720 8.37 x10^11 810 8.44 x10^11 900 7.72 x10^11 1080 6.69 x10^11
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Bacterial Growth Curve Below is a table of data from a similar experiment to what you are doing. Data Table: Incubation time (hours) OD @600nm Plate counts (CFU/ml) 0 0.18 40,000 2 0.28 53,000 5 0.53 160,000 8 0.65 218,000 12 0.94 473,000 16 1.18 700,000 20 1.41 941,000 Based on the graph constructed from the numbers above, use the indirect method to estimate the generation time for this organism under these growth conditions. Calculate the generation time for this culture by the direct method (see lab manual or text for examples). Show calculations.
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