Use this diagram as a basis for discussing and comparing the...

Use this diagram as a basis for discussing and comparing the regulation of the lac operon and the effect on the production of \beta-galactosidase when E. coli is grown under the following conditions: In the presence of high levels of lactose and absence of glucose In the presence of high levels of lactose and high levels of glucose [3] [3]

Transcript

00:01 It's a regulatory region.

00:01 The question asks you to find out, discuss, and compare the regulatory of this lac operon in the two following conditions.

00:12 Let's look at the first one.

00:14 So first of all, i put down the structure of lac gene operon on the board.

00:19 The first region is called laci.

00:21 This produces a repressor.

00:23 Then you have a cluster of lac regulatory region with a gene.

00:27 You have a cap binding site, which is in front of or upstream of the promoter region p.

00:33 The promoter region is followed by an operator region o.

00:36 It's a regulatory region.

00:38 Then you have three structure genes, z, y, and a.

00:41 Let's take a look at the first one.

00:44 The first one is the presence of high level of lactose and absence of glucose.

00:49 So in this case, let's say plus lac lactose minus glucose.

00:57 So eucalyptus prefer to use glucose as a energy source, but when the energy source glucose is not available, then it turn on this lactobacillus.

01:08 So what happened is when the repressor is expressing, it will express a repressor.

01:20 Normally without the lactose, it will actually bind to the operator region.

01:28 This will block the polymerase from moving through and produce the gene product of z, y, and a.

01:38 But with the help of lactose, which is an inducer, this inducer changed the conformation of repressor.

01:58 So you can see this is the lactose that that bind to the repressor as allos leptose.

02:05 As a result, the repressor is not able to bind to the operator region anymore.

02:11 It's open.

02:12 This is inactive or deactivated.

02:18 It can no longer bind to the operator region...

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