Using ScienceDirect - Elsevier, find the article titled "Occurrence and antibiotic resistance of multiple Salmonella serotypes recovered from water, sediment and soil on mid-Atlantic tomato farms" by SA Micallef et al. Use the article to answer the following question. Read the abstract. Which of the following antibiotics were the Salmonella strains resistant to? Select all that apply. - ampicillin - tetracycline - clindamycin - sulfisoxazole
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One strain of $E$ coli is resistant to the antibiotic streptomycin, and another strain is resistant to the antibiotic ampicillin. The two strains were cultured together and then plated on selective medium containing streptomycin and ampicillin. Several colonies appeared, indicating that cells had acquired resistance to both antibiotics. Suggest a mechanism to explain the acquisition of double resistance.
Madhur L.
The antibiotics tetracycline and kanamycin are also found on antibiotic-resistant plasmids. These antibiotics interfere with the process of translation at the bacterial ribosomes. Competent E. coli cells can be transformed with pGAL plasmids that code for tetracycline and/or kanamycin resistance, but when plated, no satellite colonies ever form, no matter how long the plates are incubated. Recalling how the ampicillin resistance works in the ampicillin-resistant pGAL plasmid, explain the absence of satellite colonies when tetracycline and kanamycin-resistant pGAL plasmids are used in transforming E. coli.
Sri K.
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