Using the memory cassette technique in the mouse, propose an experiment to confirm that the genital ridge of the early male embryo is fated to develop into the adult testis. Be as specific as possible regarding the transgenes that you propose for this experiment and how they would interact with each other. Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 125 words. (4 marks)
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Step 1
g., Sf1 or Wt1 promoter) and a reporter gene (e.g., GFP) flanked by loxP sites. Show more…
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