00:01
When we study evolutionary relationships for our understanding, we see the phylogenetic relationship between different organism.
00:16
This phylogenetic relationship is very important in establishing the evolutionary chain or the evolutionary line of sequences.
00:30
That means we can say that prokaryotes, then we have eukaryotes, then we have fungi, then we have plants and then we have animals.
00:43
This kind of evolutionary chain or evolutionary understanding comes with the help of studying phylogenetic relationships between various organism.
00:55
This phylogenetic relationships can be studied using the dna or the genetic material of different organisms and comparing them that how much dna they share, how much of their dna is same or similar and how much is coming from a similar origin while how much amount of the dna of different organisms being compared is different.
01:23
So this is a very important step, the dna study to understand the phylogenetic relationship between different organism.
01:34
Now to study the phylogenetic relationship, we perform experiments and we follow certain steps.
01:42
Therefore, firstly we have to extract the dna from the organism to be studied and lyse the cells to harvest the genomic dna.
01:55
So first we have to get the dna from the organism to be studied.
02:01
Then second we have to use pcr that is polymerase chain reaction to amplify this dna so that with a small amount of dna we can make a large amount of dna to ease our experimental studies and then we will purify this dna to obtain the r -ribosomal genes or the r -genomes...