1. The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: V0 Substrate added (mmol/min) (mmol/L) βββββββββββββ 217 0.8 325 2 433 4 488 6 647 1,000 βββββββββββββ Draw a Michels Menten plot Indicate Km and Vmax on the plot Describe the relationship between Km and Vmax Report Vmax (include correct units) Report Km (include correct units)
Added by Mireia H.
Step 1
8, 217), (2, 325), (4, 433), (6, 488), (1000, 647). Show moreβ¦
Show all steps
Your feedback will help us improve your experience
Jiva Y and 88 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Jiva Y.
Consider the data collected for an enzyme-catalyzed reaction. [S] (mM) | vβ (nM/min) 0.25 | 2.00 0.50 | 3.33 1.00 | 5.00 2.00 | 6.67 Using the data, determine this enzyme's Vmax and Km: You will need to make Lineweaver-Burk plots using Excel (or other similar spreadsheet program) and upload it to show ALL of the followings: 1. Data table given in the question. (yes, you will need to have this on your spreadsheet) 2. Table for double reciprocal values (= 1/[S] and 1/v) - so you can graph the Lineweaver-Burk plot : include units 3. Lineweaver- Burk plot (label the axis). 4. Report the values and UNITS for your Vmax and Km 5. Show your calculation/ process.
Adi S.
Below is shown kinetic data for an unknown enzyme. [S] (Β΅M) Vo (Β΅mol/s) 2.5 1.11E-4 5 2.85E-4 10 3.88E-4 25 5.81E-4 50 7.87E-4 100 8.87E-4 250 9.32E-4 500 9.58E-4 a) Using the data in the table above, make a traditional M-M kinetics plot and an L-B plot in Excel. Add a line of best fit to the L-B plot and ensure that your submission clearly shows the plots, labeled axes, and the trendline. b) What is the Vmax of this enzyme for the substrate? (to three decimal places). The units are Β΅mol s^-1, don't worry about including those here. c) What is the Km of this enzyme for the substrate? Round to the nearest whole number. The units are Β΅M, do not worry about submitting units here.
Dominador T.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD