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3. This is a group of hypothetical questions helping you think about DNA Replication. Imagine you are a synthetic microbiologist who is designing a new species of bacteria. What would happen to DNA REPLICATION in the bacterial cell if you made the following errors? Include in your response: 1) how each of the proteins or DNA sequences listed contribute to DNA replication 2) what the effect of the changes would be on replication. a. You include DNA Polymerase III, but forget to include DNA Primase. b. You include DNA Polymerase III, but forget to include DNA Polymerase I. c. You include all of the subunits of DNA Helicase except the $dnaB$. d. You forget to include the DNA Ligase in the cell. e. You remove the single-strand-binding proteins (helix-destabilizing proteins), and instead include double-strand-binding proteins (helix-stabilizing proteins). f. You forget to the $dnaQ$ gene. g. You add 10 $oriC$ sites to the chromosome, instead of 1.

          3. This is a group of hypothetical questions helping you think about DNA Replication. Imagine you are a
synthetic microbiologist who is designing a new species of bacteria. What would happen to DNA
REPLICATION in the bacterial cell if you made the following errors? Include in your response: 1) how
each of the proteins or DNA sequences listed contribute to DNA replication 2) what the effect of the
changes would be on replication.
a. You include DNA Polymerase III, but forget to include DNA Primase.
b. You include DNA Polymerase III, but forget to include DNA Polymerase I.
c. You include all of the subunits of DNA Helicase except the $dnaB$.
d. You forget to include the DNA Ligase in the cell.
e. You remove the single-strand-binding proteins (helix-destabilizing proteins), and instead include
double-strand-binding proteins (helix-stabilizing proteins).
f. You forget to the $dnaQ$ gene.
g. You add 10 $oriC$ sites to the chromosome, instead of 1.

3. This is a group of hypothetical questions helping you think about DNA Replication. Imagine you are a
synthetic microbiologist who is designing a new species of bacteria. What would happen to DNA
REPLICATION in the bacterial cell if you made the following errors? Include in your response: 1) how
each of the proteins or DNA sequences listed contribute to DNA replication 2) what the effect of the
changes would be on replication.
a. You include DNA Polymerase III, but forget to include DNA Primase.
b. You include DNA Polymerase III, but forget to include DNA Polymerase I.
c. You include all of the subunits of DNA Helicase except the dnaB.
d. You forget to include the DNA Ligase in the cell.
e. You remove the single-strand-binding proteins (helix-destabilizing proteins), and instead include
double-strand-binding proteins (helix-stabilizing proteins).
f. You forget to the dnaQ gene.
g. You add 10 oriC sites to the chromosome, instead of 1.

Added by Latoya A.

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