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4. Colony Color: Compare the color of Micrococcus luteus with the color of the other Gram-positive cocci on both the TSA and the Blood Agar plates. What differences in color do you see and can you tell the differences in color on the Blood Agar as easily as on the TSA? Part III - Preparing A Flow Chart from Biochemical Data 5. On a separate piece of paper, using the results from Table 1 above and the directions provided in Part III of this lab, prepare a flow chart identification scheme for these six Gram-positive cocci. Do not use arrangement as an identifying criterion as these can vary depending on culture conditions and slide preparation. Attach your flow chart to this Lab Report. 6. Make a generalization about the catalase results (which bacterial group(s) are catalase positive, and which are catalase negative?) 7. Which 2 species (in the same genus) are most likely to be distinguished from one another by the coagulase test and mannitol salt agar? 8. What is the significance of identifying a methicillin-resistant Staphylococcus aureus (MRSA) as the cause of a patient's infection? How might this type of infection be treated compared to a typical (non-methicillin resistant) S. aureus infection?

          4. Colony Color: Compare the color of Micrococcus luteus with the color of the other
Gram-positive cocci on both the TSA and the Blood Agar plates. What differences in
color do you see and can you tell the differences in color on the Blood Agar as easily as
on the TSA?
Part III - Preparing A Flow Chart from Biochemical Data
5. On a separate piece of paper, using the results from Table 1 above and the directions
provided in Part III of this lab, prepare a flow chart identification scheme for these six
Gram-positive cocci. Do not use arrangement as an identifying criterion as these can
vary depending on culture conditions and slide preparation. Attach your flow chart to
this Lab Report.
6. Make a generalization about the catalase results (which bacterial group(s) are catalase
positive, and which are catalase negative?)
7. Which 2 species (in the same genus) are most likely to be distinguished from one
another by the coagulase test and mannitol salt agar?
8. What is the significance of identifying a methicillin-resistant Staphylococcus aureus
(MRSA) as the cause of a patient's infection? How might this type of infection be
treated compared to a typical (non-methicillin resistant) S. aureus infection?

4. Colony Color: Compare the color of Micrococcus luteus with the color of the other
Gram-positive cocci on both the TSA and the Blood Agar plates. What differences in
color do you see and can you tell the differences in color on the Blood Agar as easily as
on the TSA?
Part III - Preparing A Flow Chart from Biochemical Data
5. On a separate piece of paper, using the results from Table 1 above and the directions
provided in Part III of this lab, prepare a flow chart identification scheme for these six
Gram-positive cocci. Do not use arrangement as an identifying criterion as these can
vary depending on culture conditions and slide preparation. Attach your flow chart to
this Lab Report.
6. Make a generalization about the catalase results (which bacterial group(s) are catalase
positive, and which are catalase negative?)
7. Which 2 species (in the same genus) are most likely to be distinguished from one
another by the coagulase test and mannitol salt agar?
8. What is the significance of identifying a methicillin-resistant Staphylococcus aureus
(MRSA) as the cause of a patient's infection? How might this type of infection be
treated compared to a typical (non-methicillin resistant) S. aureus infection?

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