00:01
Hello everyone, in this question we will be discussing about the similarities and differences in the dna replication as well as transcription.
00:11
So in the dna replication, we basically require the origin of replication from which the dna replication begins.
00:22
And here one strand of the dna acts as the template and the other strand also acts as a template and in this way the template provides the reading frame for the dna polymerase to add on the nucleotide and the nucleotide is added in the 3 ' oh direction and the nucleotide is added at the 3 ' oh end and we require the binding of primer because the dna polymerase cannot form the, cannot add the nucleotide by itself and here in transcription initiation we require the promoter sequence on which the rna polymerase binds and then after that the synthesis begins.
01:20
So here basically the point of initiation in the replication is origin of replication and here it is the promoter.
01:29
Here we require dna polymerase and here we require rna polymerase.
01:34
The similarity here is that both of them requires a dna template.
01:40
Here in the replication the template is required and here also in the transcription the dna template is required and the dna and the rna polymerase adds the nucleotide at 3 ' oh and this is also the similarity.
01:58
Here the primer is not required because the rna polymerase has the self design in such a way that it adds adenine or guanine to the sequence when it initiate the transcription.
02:14
So these are the similarities of initiation between the replication and elongation.
02:20
Now let us see the differences and similarity between the replication and transcription in the elongation step.
02:30
So here in the elongation step two fragments are formed.
02:34
One is the leading strand and other is the lagging strand and the dna polymerase 3 synthesizes the dna by adding the nucleotide to 3 ' oh and here we will see that we will have the dna template and a dna daughter strand is formed.
02:59
In the elongation step of the transcription there is no leading or lagging strand only one mrna strand in the 5 ' to 3 ' synthesis is done.
03:12
Here both of the dna strand works and that is why two strands are formed here only.
03:18
One strand that is the template strand will be red and in both of these the nucleotides are added in 3 ' oh end and here the hybrid dna and rna is formed because the dna is the template and the transcribed region is the rna.
03:42
Now talking about the termination of replication and termination of transcription.
03:53
Here the termination occurs when two of the replicating folks meet each other on the opposite side of the circular e .coli dna.
04:08
In the transcription the termination is either row dependent that is a row factor which binds to transcription termination for site and the exposed region of single stranded rna and the other termination is the intrinsic termination.
04:30
In this it depends on specific sequence in dna template strand.
04:36
So these are certain similarities and differences between transcription and replication.
04:41
And now we have been asked that the dna polymerase iii and rna polymerase iii and rna polymerase ii.
04:56
So we need to tell the similarity and difference between these two.
05:00
So both the dna and rna polymerase are the two enzymes that work upon the dna...