a person who is lactase non-persistent, the disaccharide lactose in milk is not absorbed into their small intestine as well as it is E in the small intestine of someone who is lactase persistent. Use the interactive to examine this process in Jay's and Mirai's small intestines when they consume 16 oz of milk. Then, use your observations answer the questions.Why do I feel sick when I drink milk?It must be the lactose in milk, but why does it cause symptoms in you but not in me. Which statement best describes the function of the lactase enzyme?* Lactase breaks down lactose into monosaccharides.* Lactase transports monosaccharides into the bloodstream.* Lactase builds lactose from monosaccharides.* Lactase transports monosaccharides into the intestinal cavity.
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BACKGROUND Lactase is the enzyme responsible for digestion of milk sugar in our bodies. It is a hydrolytic enzyme which breaks down the disaccharide lactose into its monosaccharide components—galactose and glucose. The lack of this enzyme in the digestive tract leads to a disorder known as lactose intolerance, a medical condition characterized by the onset of abdominal cramps and diarrhea following ingestion of foodstuffs containing lactose. This condition is virtually nonexistent among infants, but for reasons not entirely clear to medical science, becomes more prevalent after childhood. People who suffer from this condition must refrain from the consumption of dairy products or take dietary supplements of the missing enzyme in order to digest the lactose present in these foods. In this experiment, you will use a commercially available source of lactase to study the mode of action, as well as the site of physiological activity, of this enzyme. In order to follow the hydrolytic cleavage of lactose to its component monosaccharides, you will use a chromatographic method based on the oxidation of the glucose product. In turn, you will attempt to discover the site of physiological activity by varying the pH of the enzyme solution and monitoring the activity of the enzyme. The digestive enzymes of the human body are distributed throughout the digestive tract. A few, like amylase, are located in the mouth, a region of neutral pH; some are located in the stomach, a region of acidic pH, ~1; some are located in the first few inches of the small intestine, a region of near neutral pH, ~6–7; and some are located further along in the intestines, a region of slightly basic pH, ~8. By varying the pH of your test solutions to include a range of values you should be able to determine if lactase is located in the stomach, duodenum (beginning of small intestine), or further along in the intestines. The progress of the reaction of lactose as it is being hydrolyzed can be tracked by spotting a sample of the reaction mixture onto chromatographic paper at various time intervals. Once the chromatogram has been run and developed, the composition of the reaction mixture at each time point may be discerned. To provide more clarity to the results, it useful to also include a spot for the starting material (lactose) and one of the product (glucose). As you are interpreting the results of this experiment, keep in mind those principles of chromatography that were discussed in "Paper Chromatography." Mouth pH ~7 Stomach pH ~1 Duodenum pH ~6–7 Small Intestine pH ~8 FIGURE 10.1 pH Levels in the Human Body
Sri K.
In this scenario identify the enzyme and the substrate: Lactase is an enzyme that breaks down a sugar found in dairy products known as lactose. Some people are lactose intolerant, and this can be due to not having enough lactose production. People who are lactose intolerant may not feel well after eating foods containing lactose.
Adi S.
Why is the enzyme lactase necessary to digest dairy products?
Madhur L.
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