Assume you stain a bacteria by applying malachite green with heat and then counterstain with safranin and all you see are red cells, you can deduce that this bacteria does not contain.... A. archella B. mycolic acid C. capsules D. flagellin E. cell walls F. endospores
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Which of the following is NOT a common basic stain? Select one: a. Crystal violet b. Safranin c. Methylene blue d. Eosin Which of the following interpretations/conclusions would NOT be from the result/observation of red cells after a Gram-stain? Select one: a. Loss of Gram stain reaction b. Over decolorization c. Absence of decolorization step d. Gram-negative cells e. Absence of crystal violet stain step In brightfield microscopy, light passes through a specimen to make the specimen visible to someone looking through the ocular lens. Select one: a. Passes through b. Changes wavelength in c. Passes around d. Intensifies in These cells have a higher lipid content because of their outer membrane and thinner peptidoglycan: Select one: a. Merozites b. Gram-positive c. Green algae d. Gram-negative e. Endospores What allows Gram-positive cells to retain the crystal violet stain through the decolorization step compared to Gram-negative cells? Select one: a. High lipid content b. Presence of an outer membrane c. LPS d. Porins e. Teichoic acid cross-linking and thick peptidoglycan
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During the acid-fast stain, which of the following bacterial species would NOT be de-stained by the acid-alcohol solution? A) Mycobacterium tuberculosis B) Mycobacterium leprae C) Escherichia coli D) Staphylococcus aureus E) both A and B TRUE OR FALSE (Please write T or F on the answer sheet.) 41. Longer wavelength radiation improves resolution. 42. Chemical dyes are used primarily to increase resolution and not contrast. 43. The three domains of life are the Archaea, the Eukarya, and the Protista. 44. Gram-staining of bacteria provides all the physical characterization necessary to identify bacterial species. 45. A single basic dye is used in simple stains. 46. The endospore stain reveals internal structures within cells of the genera Bacillus and Clostridium. 47. Acid-fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid. 48. Specimens must be coated with metal for scanning electron microscopy. 49. Images of living specimens can be produced using atomic force microscopes. 50. Specimens are visible on a phase contrast microscope because they refract the light that illuminates them. 51. The basal bodies of Gram-positive flagella typically contain more protein ring structures. 52. The protein cores of bacterial flagella, fimbriae, and cilia are composed of a "9+2" arrangement of microtubules. 53. Many bacterial species are capable of endocytosis. 54. Not all bacteria have a cell wall. 55. Heat-fixation is a method used to limit the motility of microbes.
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