Consider the carbohydrate test results below. Carbohydrate Broth Result Glucose yellow and bubble in Durham tube Lactose dark red Sucrose yellow Based on the results from the table, explain how the TSIA tube should look for a facultative anaerobe organism. Your response depth level should be similar to the analysis in question 1, but do NOT directly copy the format and edit a few words.
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Step 1: Recall that a TSiA (triple sugar iron agar) tube is used to differentiate bacteria based on their ability to ferment sugars and produce gas and hydrogen sulfide. Show more…
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REPORT SHEET: Tests for Carbohydrates LAB: 30 Test: Water, Glucose, Fructose, Lactose, Sucrose, Starch, Unk. #X A. Benedict's Test for Reducing Sugars 1. Observations: Blue, Orange, Orange, Orange, Blue, Blue, Blue 2. Reducing/Nonreducing: Non reducing, reducing, reducing, reducing, Non reducing, No reducing, Non reducing B. Seliwanoff's Test for Ketoses 1. Colors after 1 min: No color, Pink, Red, Pink, Red, No color, Red 2. Ketose/Aldose C. Fermentation Test 1. Observations: No bubbles, Bubbles, Bubbles, No bubble, Bubbles, No bubbles, Bubbles 2. Fermentation (Yes/No) D. Iodine Test for Polysaccharides 1. Observations: Yellow, Yellow, Yellow, Yellow, Yellow, Blue black, Yellow 2. Amylose (Yes/No) Questions and Problems Q1 From the results in Part A, list the carbohydrates that are reducing sugars.
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TABLE Test for Protein using Biuret reagent Tube Contents Color reaction Conclusion 1 Distilled water 2 Starch 3 Egg albumin 4 Glucose 5 Soft drinks 2. Explain your results. Do glucose, soft drink and starch give the same results? (7 points)
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A.2. Test for Starch Using Lugol's Solution You have four tubes with content indicated in the table. The table also indicates the results. Record your conclusions regarding the presence or absence of complex carbohydrates in the last column. Table 2. Lugol's Test for Starch Tube | Contents | Initial Color Before Lugol's | Color After Lugol's Solution | Conclusion (+, - or ?) 1 | Distilled water | yellow | yellow | 2 | Glucose | yellow | yellow | 3 | Starch | yellow | dark blue | 4 | Cream | white | dark white |
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