Given an absorbance of 0.09, determine the number of bacteria in 1ml of the original sample.
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Given an absorbance of 0.05 (graph below), what is the number of viable bacteria per ml in the original sample?
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The graph provided is a standard curve. Using this, calculate the concentration of bacteria with an absorbance of 1.823 at 600 nm. Bacterial Quantitation 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 y = 6E-08x R² = 0.9949 10000000 20000000 30000000 40000000 50000000 60000000 70000000 OD at 600 nm Concentration of bacteria
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You have grown a bacterial culture to high density and wish to determine its concentration. You take a 28µL aliquot of bacteria and dilute it into 27µL of media, followed by a 1/9 dilution. The spectrophotometer reads an optical density (in Absorbance Units, A.U.) of 0.7 from your diluted sample. If 1 A.U. = 1E9 cells/mL, what was the original concentration of bacteria (in cells/mL) in the undiluted sample? NOTE: Assume that the relationship between O.D. and cell concentration is linear.
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