If a cell homogenate was subject to centrifugation at increasingly higher speeds, in which order would you expect to see these components in the pellet?
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A group of students prepares a cell homogenate from germinating peas. Next, they perform a low-speed centrifugation and collect the supernatant (Supernatant 1) and pellet (Pellet 1) fractions. The students re-centrifuge the Supernatant 1 fraction at medium speed and collect a second pellet (Pellet 2) and supernatant (Supernatant 2). Match each fraction (Pellet 1, Supernatant 1, Pellet 2, Supernatant 2) with the cellular components that are expected to be in that fraction.
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Below is a diagrammatic representation of cellular fractionation. A mixture of mechanically disrupted tissue is subjected to centrifugation. The supernatant from each centrifugation is then subjected to a subsequent centrifugation at a higher speed. 800 x g, 10 min 15000 x g, 10 min 100000 x g, 60 min 200000 x g, 3 hours Match the correct contents below (1 – 6) to the correct parts of the centrifugation procedure (A – F) during each step of the centrifugation process above. "A" refers to the contents of the entire vessel, "B" through "E" are the sedimentation pellets, and "F" is the supernatant in the final vessel: 1. Mitochondria, lysosomes, and peroxisomes 2. Homogenized cell suspension 3. Ribosomes 4. Cytosol 5. Microsomes and other small vesicles 6. Nuclei and cellular debris
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List the following cellular structures in the order that they will pellet when spun in a centrifuge (first to last): Nucleus, Amyloplast, Chloroplast, Ribosome, Mitochondria. Why do the above structures take different amounts of time to pellet during centrifugation?
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