00:02
Hello, so in this question we are going to study about the biurid test.
00:10
So the purpose of this biurid test, it is just to detect the peptide linkage, whatever the protein sample we have here.
00:25
So its main principle is of this biurate test, see, suppose we have copper sulfate plus 2 n -a -o -h when they get react it forms c -u -o -h twice plus n -a -2 -s -o -4 and this copper hydroxide this c -u -o -h twice and sample it is there protein sample then it appears the violet color appear so we can say here the reagent is, so reagent is, in this case, that is copper sulfate first and the second is the sodium hydroxide.
01:31
Right? so let us see the procedure.
01:33
See firstly we take the dry and the clean test tube.
01:40
Then add into this test tube 2 ml of the sammel of the sam.
01:48
Sample solution and then add 2 ml of biurate reagent biurate reagent that is the coso4 plus n aoh and then shake it and shake it so now firstly this 2 ml of sample solution it is taken in the test tube and 2 ml of n -a -o -h it is added and then drop by drop, coso4 added after some time color will change.
02:29
Then it is called as the positive biurid test.
02:41
Right.
02:42
So in this test tube we have coso4 plus n -a -o -h that is biurate reagent, 2ml of this.
02:53
And also we contain in this the 2 ml of the sample solution.
03:01
This bi -urit it should be poor or it should be drop by drop should be given to this test tube...