00:01
Hello everyone in this question we need to discuss about the function of rec d and d in the homologous recombination.
00:10
So for this let us first of all understand what is homologous recombination and let us take the example of single stranded break here.
00:19
So let's say this is a strand.
00:22
This is a strand in 5 prime to 3 prime direction and this is a double stranded dn a so here we have another homologous double -stranded dna.
00:35
In this, endonuclease will form a nick at both the dna strands.
00:45
So now there is a nick created.
00:50
After this, there will be strand displacement.
00:54
And then these strands will invade and with the help of the dna polymerase will be.
01:00
Use this strand as the template and will add the nucleotides.
01:04
And similarly here also the nucleotides will be added.
01:08
So this here is known as the holiday junction.
01:14
And then after that branch migration takes place so that there is complete exchange of the dna.
01:22
And then the holiday junction is solved in which first of all there is duplication in which there is 180 degree rotation.
01:33
So after this we will have the strands like this, which after the 180 degree separation, after the 180 degree rotation, it will look something like, which is known as the kai form and then after this the vertical split or the horizontal split horizontal split takes place which forms the recombination which forms the recombinant d and a...
