00:02
So you're looking at a figure of gel electrophoresis.
00:05
So you know that it's a lydian dna, and the dna is digested by two different enzymes, cell 1 and hene3.
00:12
So according to the figure that the cell 1 produce 2 5kb fragment and this is by itself, and the hene3 by itself cut into 5kb and 2 2 .5kb band.
00:42
Then if you have a double digestion, then you produce four different fragment each 2 .5.
01:04
So first of all, let's say, a, what is the total size of dna fragment? so the total size obviously is gonna be all the sequence add up together, all the fragment add up together.
01:18
So the total size is gonna be five plus five, which is 10kb long.
01:27
So this is a total.
01:29
So you can see if you add those fragment together, they all equals 10kb.
01:38
In the linear line, draw the restriction map.
01:44
So let's say this is the 10kb band, and cell 1 cut the 10kb band into a 2 5kb fragment.
01:57
That means that it cut right in the middle.
02:02
So you have 5kb and 5kb on each side...