List possible ways to track the presence of a protein during its purification. You have several cell lysates and want to determine which of them contain
Added by Jose Antonio R.
Close
Step 1
Step 1: To track the presence of a protein during its purification, several methods can be employed, often relying on the protein's unique physical, chemical, or biological properties. Show more…
Show all steps
Your feedback will help us improve your experience
Parminder P and 62 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Last week, you analyzed your subcellular fractions to determine the total amount of protein in each fraction. Proteins that are found exclusively in association with certain organelles can act as markers. You will be using COX6 as a marker for mitochondria. Now you need to separate the proteins in each fraction so that next week you can visualize COX6. Propose a series of experimental techniques that you could do to separate the proteins present in each fraction and allow detection of COX6 visually. Justify your technique choices.
Parminder P.
3. Suppose you were given an unknown organism that was dredged out of the mud from the bottom of the ocean. You were able to grow the organism, and you used typical methods to break open the cells and release the soluble proteins into a buffered solution. You than centrifuge the solution to remove insoluble cell debris. b. Using SDS-PAGE, you discover an abundant protein in this mixture. You isolate and purify a significant quantity of this protein and use standard analytical procedures to try and identify the protein. There comes a point in your analysis that you strongly believe that the protein is a protease, even though you have not tested its enzymatic activity against any proteins. Considering that this putative protease is from an unknown organism, what kind of evidence would allow you to come to this conclusion?
Adi S.
A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in a solution. Only three other proteins (A, B, and C) are present. The proteins have the following properties: pI (Isoelectric point) Size (Mr) Bind to DNA protein A 7.4 82,000 yes protein B 3.8 21,500 yes protein C 7.9 23,000 no protein X 7.8 22,000 yes What type of protein separation techniques might the biochemist use to separate. (a) protein X from protein A? (b) protein X from protein B? (c) protein X from protein C?
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD