Looking specifically at the a17183e antibody results what...

Name: looking specifically at the a17183e antibody results what can you conclude about the specificity of this particular antibody select all that apply looking specifically at the a17183e antibod 09358
Uploaded: 2022-05-04T15:27:35-08:00
Duration: 8 min 23 s
Channel: Suman K
Description: looking specifically at the a17183e antibody results what can you conclude about the specificity of this particular antibody select all that apply looking specifically at the a17183e antibod 09358

Looking specifically at the A17183E antibody results, what can you conclude about the specificity of this particular antibody? (select all that apply) Looking specifically at the A17183E antibody results, what can you conclude about the specificity of this particular antibody? (select all that apply) It is conformation specific There are sequence differences between the M and DO forms of \alpha -synuclein It does not recognize the amino acids found in the M form There are sequence differences between the M and HO forms of \alpha -synuclein It recognizes only the DO oligomers

Transcript

00:01 So here it is given to us that our map is developed through different antibodies that bind distinct regions of the same surface exposed protein from an emerging virus.

00:11 Now if we talk about antibody x, then it binds to a linear epitope within the small n -terminal transmembrane.

00:31 Now if we talk about the antibody y, then this antibody y binds to some discontinuous or we can say conformational epitope in the large water soluble c terminal domain.

01:14 Now accordingly with this information we have been asked that which antibody would be expect to perform better for the following application.

01:26 And here our correct answer will be antibody why that is this antibody will perform better as compared to both of them.

01:38 If we talk about this linear epitope then this linear b cell epitopes are composed of.

01:45 Contiguous residues and because of this contiguous residues they are not able to perform better as compared to the antibody vibe and if we talk about antibody why which binds to the conformational epitope then this conformational epitope is important form of epitope which is found by the residues that are sequentially discontinuous and they are also close together in 3d spaces now we have been given with three statements and have been told to explain our reasoning focusing on the silent information for each of them.

03:04 And here our first statement is telling that specific detection of the viral protein by sds page, which is coupled with western blot.

03:23 So if we talk about this statement, then western blot is referred with sds page because a few reasons which are that it quote the hydrophobic region of the protein with its negative charge and it also overcome the overall positive charge of the protein so that the protein can migrate to a towards the positive electrode.

04:38 Now talking about the second statement then it is telling that immunofinity purification of the protein under native conditions.

05:00 So here we are talking about immunoaffinity chromatography and this immunoaffinative chromatography is also known as iac, which combines the use of lc with the specific bandies of antibodies or related agents...

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