Question

1) List three ways you can burst open an animal cell. (2 points) 2) List two features of a molecule you can use to separate it away from molecules of other types. Underline the one you used in lab. (2 points) 3) Think about the columns you used in lab. Why doesn't the DNA leave the column until you add the elut ion buffer? (2 points) 4) For each of the steps of replication below, explain how this is accomplished during PCR (0.5 points each) a. Hydrogen bonds between DNA strands are broken. b. 3'OH groups are provided, so that DNA polymerase can extend the chain 5) In this experiment, we only want to amplify a small section of DNA. How do we make sure only a specific section of DNA is copied, rather than a random piece? (2 points) 6) List the steps of the PCR reaction and describe what happens at each step.

          1) List three ways you can burst open an animal cell. (2 points)
2) List two features of a molecule you can use to separate it away from molecules of other types. Underline
the one you used in lab. (2 points)
3) Think about the columns you used in lab. Why doesn't the DNA leave the column until you add the
elut ion buffer? (2 points)
4) For each of the steps of replication below, explain how this is accomplished during PCR (0.5 points each)
a. Hydrogen bonds between DNA strands are broken.
b. 3'OH groups are provided, so that DNA polymerase can extend the chain
5) In this experiment, we only want to amplify a small section of DNA. How do we make sure only a specific
section of DNA is copied, rather than a random piece? (2 points)
6) List the steps of the PCR reaction and describe what happens at each step.

1) List three ways you can burst open an animal cell. (2 points)
2) List two features of a molecule you can use to separate it away from molecules of other types. Underline
the one you used in lab. (2 points)
3) Think about the columns you used in lab. Why doesn't the DNA leave the column until you add the
elut ion buffer? (2 points)
4) For each of the steps of replication below, explain how this is accomplished during PCR (0.5 points each)
a. Hydrogen bonds between DNA strands are broken.
b. 3'OH groups are provided, so that DNA polymerase can extend the chain
5) In this experiment, we only want to amplify a small section of DNA. How do we make sure only a specific
section of DNA is copied, rather than a random piece? (2 points)
6) List the steps of the PCR reaction and describe what happens at each step.

Added by Rita C.

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