Researchers have been able to use modified CRISPR/Cas9 systems to study the role of histone modification in gene expression for DNA sequences that are complementary to the CRISPR guide RNA. Variants of Cas9 without the ability to cleave DNA and an additional histone acyltransferase domain (dCas9-HAT) have been successfully engineered and expressed as proteins in laboratory organisms. Which of the following is the most likely impact on gene expression when a nearby DNA sequence is targeted by a dCas9-HAT construct?
Group of answer choices:
The guide RNA will cause the dCas9-HAT construct to avoid the DNA sequence and H3K27 acetylation will remain the same.
The guide RNA will cause the dCas9-HAT construct to bind the DNA sequence, H3K9 trimethylation will decrease, and transcription will decrease.
The guide RNA will cause the dCas9-HAT construct to bind the DNA sequence, H3K27 acetylation will increase, and transcription will increase.
The guide RNA will cause the dCas9-HAT construct to bind the DNA sequence, H3K9 trimethylation will increase, and transcription will increase.