Texts aug uuc uau acg cgg cuc aaa acu uaa what would happen...

Name: texts aug uuc uau acg cgg cuc aaa acu uaa what would happen if a single base mutation occurred which changed the sixth nucleotide in the above mrna sequence to uracil instead of cytosine suc 69097
Uploaded: 2023-05-27T04:19:42-08:00
Duration: 15 min 47 s
Channel: Jenny Wu
Description: texts aug uuc uau acg cgg cuc aaa acu uaa what would happen if a single base mutation occurred which changed the sixth nucleotide in the above mrna sequence to uracil instead of cytosine suc 69097

Texts: AUG UUC UAU ACG CGG CUC AAA ACU UAA What would happen if a single-base mutation occurred which changed the sixth nucleotide in the above mRNA sequence to uracil instead of cytosine such that the new sequence would be: AUG UUU UAU ACG CGG CUC AAA ACU UAA What would happen if a single-base mutation occurred which changed the sixth nucleotide to guanine instead of cytosine such that the new sequence would be: AUG UUG UAU ACG CGG CUC AAA ACU UAA

Transcript

00:01 So i copy a sequence on a board.

00:04 Identify the type of mutation and how they would affect the protein made if the following change occur in the dna antigenous strand.

00:12 So very first thing you want to write down the sense strand.

00:17 So it follow base pair rule a t g t g g t g c c g a t g a.

00:41 Now let's go ahead and transcribe this sense strand into mrna.

00:50 The mrna is going to be the same sequence as the sense strand and complementary to the antisense.

01:03 So rna does not have a t.

01:06 So whenever you have a t in the sense strand, it will be replaced by a u.

01:10 So mrna is going to be a.

01:13 Now the second one is a t in dna.

01:15 It's going to be a u in mrna.

01:17 And then u g g.

01:21 The rest of the sequence are the same as the dna.

01:35 Now once we have the dna, we can translate the mrna into a polypeptide by using a code on chart.

01:44 So i have already found the code on chart online, but you can always found them from code on table or chart from the book, or you can search for code on table.

01:55 So basically it tells you which code i'll give you what amino acid specifically.

02:01 Now let's first of all translate this particular mrna into a polypeptide.

02:08 Now the first one is a u g.

02:10 It's a star code on.

02:12 It's where the ribosome start translate the mrna.

02:18 It only start a stem for a particular amino acid methionine.

02:23 The second one is u g g.

02:26 It's stand for a tryptophan trp.

02:33 The next one u g c stem for assisting the next one c g a stem for a rna.

02:49 And the last one u g a is a stop code.

02:51 It does not give you a particular amino acid, but it tells the ribosome that this is the end of the polypeptide and you need to take off.

02:59 So this is the wild type, i guess, polypeptide.

03:06 So now, since we have finished that, let's take a look at all the mutations.

03:11 So the first mutation says that first code on change from tac to tat.

03:22 So let's say if it now become a tat, the rest of them are the same.

03:32 So this means that the sense strand is now going to become a a u.

03:47 So as you can see, the original code is a u g.

03:53 Now it become a u a.

03:55 So basically the first methionine is gone.

03:58 So when you have a polypeptide, when the methionine, the star code is mutated, most likely the translate entire polypeptide because methionine, the first amino acid is where the ribosome start to translate the mrna.

04:20 So this actually may cause the star code on mutation is a very severe mutation.

04:32 Maybe the entire, most likely the entire polypeptide will not be translated because the star code on is gone.

04:42 So no polypeptide is made.

04:54 So this is the first type of mutation.

04:57 The second type of mutation is the third code on change from acg to aca.

05:05 So now the antisense third one now is acg to aca.

05:20 So i'm going to just put the wrong one here.

05:27 So this cause the sense to be u g.

05:37 So this is the, um, the a here is in antisense.

05:45 So ugu is going to be the code on sense.

05:49 So if we check our code on chart, um, a ugu is going to be a assisting as well.

05:58 So the rest of the sequence is going to be the same.

06:04 So it's still going to be methionine not changed tryptophan, cysteine, arginine.

06:15 And then the last one is still the stop code on.

06:18 So as you can see, you start out with assisting and you end up with assisting, although the code on itself is this, uh, is different.

06:26 It does not cause a change in amino acid.

06:29 So this type of mutation is called the silent mutation, which means that the dna change or mrna change doesn't cause a change in the polypeptide sequence change.

06:50 They have the same amino acid sequence.

06:53 So it's a silent mutation.

06:56 The next mutation is that the third code on change for, uh, the nice nucleotide, uh, change from g to t.

07:04 So let's take a look at the ninth.

07:09 So this one now goes from, uh, g to t.

07:14 So three.

07:16 Now you, you go from a, a c g to become a, a c t.

07:25 All right.

07:26 So now let's write down the, um, this is the antisense...