00:01
Hello students, the question is regarding the phalloidin that cause the, that can be used to fluorescently labelled actin filament with a cell.
00:15
We have to describe how this is possible considering that the phalloidin itself is not fluorescent, its purpose, what is the purpose of the fixation state and all.
00:30
If we see phalloidin, it is a toxin produced by death cap mushroom named amanita phalloidin or phalloidis.
01:12
It binds to the actin, actin filament with high affinity and specificity and stabilize them against, against depolarization, depolymerization, depolymerization.
01:50
This makes it useful tool for labelling the actin filaments, labelling the actin filament.
02:07
If we see itself it is not fluorescent, this phalloidin, so if we see, so it needs to be conjugated to a fluorophore in order to visualize under the microscope.
02:27
This is usually done by attaching a fluorescent dye, fluorescent dye to the phalloidin molecule which can be excited by specific wavelength of light and emit different wavelength of light that can be detected by the camera or other imaging device.
03:07
The fixation step if we see, it is necessary to preserve the structure, fixation step if we see, it is necessary to preserve the structure of the cell and prevent them from dragging or changing over the time...
