The polymerase chain reaction (PCR) All of these are...

The polymerase chain reaction (PCR) All of these are correct. can create messenger RNA molecules from small pieces of DNA. enables researchers to create huge numbers of copies of tiny pieces of DNA. utilizes RNA polymerase to build strands of DNA. enables researchers to determine the sequence of a complementary strand of DNA when they have only single- stranded DNA.

Transcript

00:03 Hello everyone.

00:07 The topic for analysis in this segment is polymerous chain reaction.

00:15 The question is, what is the primary purpose of polymerous chain reactions? what is this process used for? firstly, scientists, when looking for bacteria or viruses that have a particular trait, the trait coming from their dna, their deoxyribiniclac acid, within these microscopic organisms, must go through a process of separating the segment of dna that they suspect has the ability to give that particular trait.

01:04 So, when going through this process, where does the use of polymerous chain reactions come in, and what is it used for? first, scientists must go about separating this segment of dna, the dna that they are seeking to identify or think that it is.

01:32 Then a polymerous chain reaction is related to amplifying or multiplying this segment of dna.

01:46 It's a little bit more intricate than just saying.

01:54 So for illustrative purposes, we have a dna segment, all right, and our dna it's double -stranded.

02:06 And we have nucleotide base pairs, one on either side but matching.

02:17 So if this is dna, then we're dealing with denine and thymine as well as cetacein and guanine.

02:24 And so we know that this is what's going on in our dna.

02:31 So there's a segment of the dna that is of interest, and scientists use something that's called restriction enzymes to excise or cut out the piece of interest.

02:52 So as an example, that restriction enzyme would bind to the nucleotide -based pairs that it matches.

03:05 So let's say, for instance, it recognizes a, a, a, a, satycin and guanin.

03:16 That is just before an adenicin and thiamen and just before another satycin and guanin and so it cuts that out.

03:29 But normally they cut these out in three segment, three rather base pairs of three sets.

03:40 So they would excise something like right there.

03:45 Let's use a different color and it can be any number doesn't have to be three but normally it's a set of nucleotide base pairs and it would do that also on another end so let's say that would happen here and it would excise all right now after they have restricted or taken out the segment of interest that new piece that is created would look something like this.

04:27 So we're going to try to mimic what we have cut out...

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