What can be said of the restriction sites of the crime scene and suspect DNA samples?
Added by Julia B.
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This involves using restriction enzymes that cut DNA at specific sequences, allowing for the analysis of the fragments produced from both the crime scene and suspect DNA samples. Show more…
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Adi S.
Post Lab: Interpretation of Results The figure on the right shows the banding patterns of six DNA samples being investigated: CS (Crime Scene) S1 (Suspect 1) S2 (Suspect 2) S3 (Suspect 3) S4 (Suspect 4) S5 (Suspect 5) 1. What are we trying to determine? Restate the central question. band patterns in order to catch the suspect by comparing different DNA 2. Which of your DNA samples were fragmented? What would your gel look like if the DNA were not fragmented? all dna were fragmented. if it wasn't fragmented then we would see single band 3. What caused the DNA to become fragmented? Restriction enzyme 4. What determines where a restriction endonuclease will "cut" a DNA molecule? each restriction enzyme has a specific sequence which it recognizes and cuts. 5. A restriction endonuclease "cuts" two DNA molecules at the same location. What can you assume is identical about the molecules at that location? 6. Do any of your suspect samples appear to have EcoRI or PstI recognition sites at the same location as the DNA from the crime scene? 7. Based on the above analysis, do any of the suspect samples of DNA seem to be from the same individual as the DNA from the crime scene? Describe the scientific evidence that supports your conclusion:
Sri K.
If cells left at the crime scene by the perpetrator produced the banding pattern in lane 2, which of the known suspects (in lanes 3-9) is the perpetrator? How can the use of different restriction enzymes produce different DNA fingerprints for the same person?
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