What is the most likely outcome if the decolorization step...

Name: what is the most likely outcome if the decolorization step is omitted during the gram staining process a both gram positive and gram negative bacteria will appear purple b gram positive bact 24672
Uploaded: 2023-02-03T07:22:33-08:00
Duration: 4 min 52 s
Channel: Madhur L
Description: what is the most likely outcome if the decolorization step is omitted during the gram staining process a both gram positive and gram negative bacteria will appear purple b gram positive bact 24672

What is the most likely outcome if the decolorization step is omitted during the Gram staining process? A Both Gram-positive and Gram-negative bacteria will appear purple. B Gram-positive bacteria will appear pink, and Gram-negative bacteria will be colorless. C Gram-negative bacteria will appear colorless, and Gram-positive bacteria will be faintly pink. D Both Gram-positive and Gram-negative bacteria will appear pink.

Transcript

00:01 Hello student here the question is regarding the gram staining and we know these gram staining is done in case of bacteria and these gram staining based on the mainly the peptidoglycan of the cell wall so we know in case of the gram positive bacteria so these gram positive bacteria having the thick layer of peptidoglycon and and these thick layer of peptidoglycon have the capacity to retain these gram staining.

00:40 And in case of gram negative bacteria, there are the thin layer of these peptidoglycon.

00:48 And it is also porous in nature.

00:52 So if we look into the procedure of the gram staining, then first we use.

00:58 So if we talk about the gram staining procedure, then in this process, then in this process, first we take fresh shell and these fresh shell are kept at the slide and fixed into this and after fixing into the slide the crystal violet stain is used for the 30 to 40 second in order to stain and rinse off the excess one.

01:32 Rinse water to remove excess.

01:37 A sustain.

01:41 So after doing this, these crystal violet is going to fix into this fresh cell.

01:50 So these cell become the purple.

01:54 And after these getting the purple, there are the gram iodine solution is added.

02:03 These solution is making a complex with the crystal violet.

02:09 So here the iodine and these crystal violet containing cell make a complex and this complex is still purple in color.

02:26 So up till now these are the purple color...

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