What is the role of the Gram stain reagents in the staining process.
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This primary stain penetrates the cell walls of all bacteria, staining them purple. Show more…
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5. What is the function of a mordant, and which reagent serves this purpose in the Gram stain procedure? 6. List the reagents of the Gram stain technique in order and their general role in the staining process. 7. In what type of cell, gram-positive or gram-negative, would you find lipopolysaccharide in its cell wall? Matching Questions Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells. Consult your chart at the beginning of this lab report if you need help remembering the correct Gram reaction for each species. Choices may be used more than once. 1. Staphylococcus aureus before the primary stain 2. Pseudomonas aeruginosa after the primary stain 3. Bacillus megaterium after the addition of the mordant 4. Staphylococcus aureus after decolorization 5. Moraxella catarrhalis after decolorization 6. Pseudomonas aeruginosa after decolorization 7. Bacillus megaterium after adding the counterstain 8. Escherichia coli under the microscope if you forgot to apply safranin 9. Escherichia coli under the microscope if you forgot to apply decolorizer 10. Bacillus megaterium under the microscope if you forgot to apply iodine
Adi S.
Read the steps of the Gram stain technique in the introduction to Procedure 10.2. Explain the role of each of the reagents used in the Gram Stain. - Primary Stain (Crystal Violet) - Mordant (Gram's iodine) - Decolorizing Agent (Acetone-alcohol) - Counterstain (Safranin) 2. At the end of the Gram stain procedure, what color will each of the bacterial types be? - Gram-positive: - Gram-negative:
Q3. A) Reorder according to the Gram stain use. 1- Mordant: Iodine. 2- Crystal Violet Staining Reagent. 3- Counterstain: Safranin 4- Decolorizing Agent (Ethanol) B) Which part of a bacteria cell wall "holds" the stain? Q4. What is the clinical significance of the Gram stain?
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