what is the size of the plasmid explain different banding patterns you see in lanes 2, 3, and 4
Added by Bego-A S.
Step 1
- To determine the size of the plasmid, you would typically compare the bands observed in the gel electrophoresis to a DNA ladder or marker that has known sizes. By measuring the distance traveled by the plasmid band and comparing it to the ladder, you can Show more…
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What is the correct order of the DNA bands (in terms of molecular size) from Lanes 2-4 on the figure?
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Observe the photograph of the stained gel containing your sample and those from other students. Orient the photograph with the sample wells at the top. Interpret the band(s) in each lane of the gel: a. Scan across the photograph to get an impression of what you see in each lane. You should notice that virtually all student lanes contain one or two prominent bands. b. Now locate the lane containing the pBR322/BstN 1 markers (See ladder to the right). Working from the well, locate the bands corresponding to each restriction fragment: 1857 bp, 1058 bp, 929 bp, 383 bp, and 121 bp (the last band may be faint). Look at the other marker lane also – 100 bp ladder. c. The amplification product of 440 bp should slightly above the 383-bp marker. d. It is common to see a second band lower on the gel. This diffuse (fuzzy) band is “primer dimer,” an artifact of the PCR reaction that results from the primers overlapping one another and amplifying themselves. Primer dimer is approximately 50 bp, and should be in a position below the 121-bp marker. A good PCR reaction will only have a single band at the expected size with possible primer dimer bands. e. Additional faint bands, at other positions on the gel, occur when the primers bind to chromosomal loci other than mt control region and give rise to “nonspecific” amplification products.
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