what size bands should the pcr produce using 16s primers 1000 10000 2500 1600 04349

Name: what size bands should the pcr produce using 16s primers 1000 10000 2500 1600 04349
Uploaded: 2022-10-19T22:11:31-08:00
Duration: 2 min 6 s
Channel: Rebecca Clements
Description: what size bands should the pcr produce using 16s primers 1000 10000 2500 1600 04349

Step 1: The question asks about the expected size of PCR bands when using 16S primers. This refe

What size bands should the pcr produce using 16s primers...

Imagine you are in the lab, setting up your first PCR reaction. You want to PCR amplify a small region of DNA. The template is shown below. Only one DNA strand is shown, you can draw the other strand if it helps. 5’-GGCACCTGGACTTAGCACTGACAGTGAAAGTTTTTTCCCCAGTCAGTGGTTAACG-3’ Here are eight PCR primer sequences that you have already. Circle the two primers that you would use to amplify from this region of DNA. (12 points) 5’-TGGACCTGAATC-3’ 5’-GTCAGTGGTTAAC-3’ 5’-CTAAGTCCAGGT-3’ 5’-GTTAACCACTGAC-3’ 5’-GATTCAGGTCCA-3’ 5’-CAATTGGTGACTG-3’ 5’-ACCTGGACTTAG-3’ 5’-CAGTCACCAATTG-3’ Using your PCR primers in a PCR reaction with the template DNA, what is the size of the PCR product you expect after 20 cycles of PCR, in basepairs? Write the number in the box. (7 points) If you start with 5 molecules of doublestranded DNA, how many molecules of DNA do you expect to have after 5 cycles of PCR? Write the number in the box. (3 points)

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