00:01
You're going to answer this question, let's talk about gram staining.
00:03
Let's suppose here you have your gram positive and your gram negative.
00:07
Here are your crystal violet.
00:11
The first step of gram staining is to apply crystal violet.
00:14
It is a primary staining and it is going to stain both type of bacteria as purple.
00:22
It means both this crystal violet is going to stain the cell wall of both gram positive and gran positive.
00:29
The second step is to apply iodine.
00:32
This iodine is the more than it is going to fix the crystal violet to the peptidoglycan layer of the cell wall of this bacteria.
00:41
So they are going to remain purple.
00:45
Now the third step is to apply alcohol.
00:49
Remember that the peptidoglycan layer of gran postydele bacteria is very thick.
00:54
So once you have applied the iodine, this peptidoglycan layer is going to be, or this alcohol is not going to be able to decolorize the peptidoglycan layer because because of the thickness of this peptideolicin ligating grand positive bacteria.
01:11
So they are not going to be able to decolorize gram positive and they are going to remain purple...