When performing an Antibody Screen or an Antibody ID Panel, during the AHG phase why are the rbcs in the tubes washed 3 times before adding AHG reagent? Option A To wash away any extra antibodies which are not bound to rbc antigens Option B To wash away any extra rbc antigens which are not bound to antibodies Option C To wash away any extra AHG reagent Option D To wash away any residual EDTA from the patient’s rbcs
Added by Joyce T.
Step 1
Therefore all unbound antibodies present in the patient serum must be removed before adding the AHG reagent. Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 58 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Adi S.
1) Why do we need a standard in our ELISA experiment? A. To create a standard curve B. To ensure a high detection signal C. To validate the false negative D. To avoid a false positive 2) What is the purpose of agitating an ELISA plate? A. Optimizing the signal-to-noise ratio B. Reducing the washing time C. Increasing the rate of binding D. Prolonging the incubation time
Marlyn J.
Marybeth R., a 27-year-old female pregnant with her second child, had routine orders for a "type and screen," with the following results: ABO/Rh (tube method) Patient RBCs (forward type) Patient RBCs (reverse type) Anti-A Anti-B Anti-D A cells B cells 3+ 0 4+ 0 4+ Antibody screen (tube LISS method) IS 37°C AHG CC SC1 0 0 0 4+ SC2 0 2+ 3+ SC3 0 2+ 3+ Autocontrol 0 0 0 4+ Abbreviations: IS, immediate spin; AHG, antihuman globulin; CC, check cells What is Marybeth's ABO and Rh blood type? What would be the interpretation of the antibody screen? What immunoglobulin class is the most probable for this antibody? Is the antibody an alloantibody and/or autoantibody? Can either be ruled out? Explain. What detail in the patient history would provide further evidence for your answer to question 4? What procedure would the technologist perform next? How are antibodies ruled out in the cross-out method of antibody panel interpretation? Explain the procedure in two to three sentences.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD