00:01
Hello everyone in this question we will be talking about the lack operon which is an inducible operon.
00:08
So here if we draw the construct of the lack operon we will see that here the promoter region is present the operator region is present and then we have the lack i gene and then we have the promoter region operator region for the lack operon gene.
00:28
So the lag genes are laxed, laxed, lax y, and lack a gene.
00:36
The rna polymerase binds to the promoter region, and then it will transcribe this lack i gene.
00:47
And so here the repressor will be formed and this repressor here is active repressor.
00:55
And this will bind to the operator and due to which the repressor.
00:59
Transcription will be of and this happens when the lactose concentration is low and the glucose concentration is high and this glucose basically inhibits the adenylylyl cyclase due to which the atp to cyclic amp conversion cannot take place and due to this the catabolic activiator protein that is the camp receptor protein crp that binds to the upstream region of the promoters.
01:38
So here the crp and cyclic amp binds but this cannot take place because the camp here will not be formed due to the glucose high concentration.
01:50
But if glucose concentration is low and lactose concentration is high then this hair will be formed and due to which due to which the crp cam complex will bind to the upstream region of the promoter.
02:05
And the allosteric form of the lactose, that is the allolactose, will bind to the repressor and due to which now it is in its inactive form...