00:01
Hello everyone, in this question we have been said that who was the cat criminal and how do you know? so, basically here if a researcher wants to know who was the cat criminal, then researcher must have designed an experiment which includes the laboratory examination.
00:22
Researcher determine if a human or other animal committed the fictional crime of a scaring a cat.
00:28
So, researcher begin this by performing an in silico pcr using provided other animal and human specific pcr primers to determine the sequence to be amplified and predict pcr amplicon sizes.
00:47
So, after that they then blast that is the basic local alignment search tool is used.
00:55
So, the in silico pcr result is used to confirm that the pcr primers are designed to amplify the genomic fragments of the cardiac actin gene in both that animal and humans.
01:09
So, finally, they use the dna quantification techniques.
01:15
The pcr as well as the agarose gel electrophoresis is used to define to confirm the culprit and they confirm the results by analyzing the sanger sequencing...