Question

You learned that after transformation, bacteria that successfully took up a plasmid can be selected using antibiotic resistance or another "selection marker" on the plasmid. Let's say that other selection marker were an auxotrophic marker (gene), where auxotrophic markers encode proteins that allow cells with the marker to survive without a certain essential nutrient in the medium because they can now make that nutrient themselves, whereas they couldn't make it on their own prior to transformation. The researcher could then put the newly-transformed bacteria onto an agar plate that lacked that nutrient. Would that system, as I've described it, allow for selection of transformants? ◻ Yes ◻ No

Name: you learned that after transformation bacteria that successfully took up a plasmid can be selected using antibiotic resistance or another selection marker on the plasmid lets say that other 31535
Uploaded: 2023-02-10T08:07:14-08:00
Duration: 2 min 46 s
Channel: Madhur L
Description: you learned that after transformation bacteria that successfully took up a plasmid can be selected using antibiotic resistance or another selection marker on the plasmid lets say that other 31535

          You learned that after transformation, bacteria that successfully took up a plasmid can be selected using antibiotic resistance or another "selection marker" on the plasmid. Let's say that other selection marker were an auxotrophic marker (gene), where auxotrophic markers encode proteins that allow cells with the marker to survive without a certain essential nutrient in the medium because they can now make that nutrient themselves, whereas they couldn't make it on their own prior to transformation. The researcher could then put the newly-transformed bacteria onto an agar plate that lacked that nutrient. Would that system, as I've described it, allow for selection of transformants?
◻ Yes
◻ No

you learned that after transformation bacteria that successfully took up a plasmid can be selected using antibiotic resistance or another selection marker on the plasmid lets say that other 31535

Added by Kelly A.

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